Abstract
For the successful functioning of a breeding and nursery center of scientific and practical work with fruit and small fruit crops, an important task is to create repositories, including thosein the field. A field repository is a plant gene bank based in accordance with international standards on planting material that is free from dangerous pathogens, including viruses, representing tested for productivity typical plants.For the purpose of a comparative study of promising varieties, hybrids and clones-candidates for original plants, a field repository and mother plantation of strawberries clones and varieties have been created on the territory of the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery.As a result of research in 2015-2020, 386 high-yielding strawberry plants were selected and tested for the main harmful viruses using diagnostic kits from “Loewe” firm (Germany). The prevalence of harmful Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Tomato black ring virus (TBRV), Strawberry latent ringspot virus (SLRSV), Cucumber mosaic virus (CMV) in strawberry plantations depended on the area cultivation, varietal composition of plantings and ranged from 31 to 69 %. The prevalence of viruses RpRSV (up to 36 %), TBRV (up to 31 %) and CMV (up to 22 %) was established. The high efficiency of dry-air thermotherapy for the recovery of strawberries with the number of virus-free intact plants of 56 % has been shown.A genebank of "candidates for original plants" has been formed from 234 strawberry plants of 39 varieties and hybrids, which, after confirming their status by PCR, will be transferred to the category of "original plants".
Highlights
Авторы благодарят научного сотрудника ФГБНУ ФНЦ Садоводства Ю
A field repository is a plant gene bank based in accordance with international standards on planting material that is free from dangerous pathogens, including viruses, representing tested for productivity typical plants.For the purpose of a comparative study of promising varieties, hybrids and clones-candidates for original plants, a field repository and mother plantation of strawberries clones and varieties have been created on the territory of the Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery.As a result of research in 2015-2020, 386 high-yielding strawberry plants were selected and tested for the main harmful viruses using diagnostic kits from “Loewe” firm (Germany)
The prevalence of harmful Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Tomato black ring virus (TBRV), Strawberry latent ringspot virus (SLRSV), Cucumber mosaic virus (CMV) in strawberry plantations depended on the area cultivation, varietal composition of plantings and ranged from 31 to 69 %
Summary
Для успешного функционирования селекционно-семеноводческого центра в области научно-практической работы с плодовымии ягодными культурами важной задачей является создание репозиториев, в том числе в полевых условиях. Полевой репозиторий – банк генофонда растений, базирующийся в соответствии с международными стандартами на свободном от опасных патогенов, в том числе вирусов, посадочном материале, представляющем собой типичные проверенные на продуктивность растения. С целью сравнительного изучения перспективных сортов, гибридов и клонов-кандидатов в исходные растения на территории Федерального научного селекционно-технологического центра садоводства и питомниководства созданы полевой репозиторий и маточник клонов и сортов земляники садовой. Показана высокая эффективность суховоздушной термотерапии для оздоровления земляники с выходом свободных от вирусов интактных растений 56 %. Сформирован генобанк «кандидатов в исходные растения» из 234 растений земляники садовой 39 сортов и гибридов, которые после подтверждения их статуса методом ПЦР будут переведены в категорию «исходное растение». Авторы благодарят научного сотрудника ФГБНУ ФНЦ Садоводства Ю. Поступила: 28.05.2021 Принята к публикации: 30.08.2021 Опубликована онлайн: 27.10.2021
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