Features of reproduction in vitro of Silene cretacea and Silene hellmannii

Abstract

This article provides information about improving of the clonal micropropagation technology and in vitro reproduction peculiarities of Silene cretacea and S. hellmannii. As a result of the research, optimization of cultivation conditions at different stages of clonal micropropagation of S. cretacea and S. hellmannii cultures was carried out. The optimal mode of sterilization seeds of species S. cretacea and S. hellmannii has been selected - a 5 % solution of Lysofornin® 3000 at an exposure of 7 minutes. The maximum germination of S. hellmannii seeds was 75 % observed on day 11 of the experiment, and for S. cretacea 50 % on day 14. At the same time, the germination percentage of S. hellmannii is 30 % higher than that of S. cretacea. The highest reproduction coefficient for S. cretacea and S. hellmannii was observed when using Cytodef at a concentration of 0.5 mg/l. It was 23.2 ± 0.7 and 26.2 ± 0.4, respectively. As a result of the conducted research, optimal conditions for the long-term preservation of regenerating plants have been selected. Plants are stored in vitro in a climatic chamber SANYO MRL-351H on a nutrient medium of У MS supplemented with 6-BAP 0.4 mg/l, sucrose (20 mg/L), t (3-7 °C) and illumination (500-1200 lk).