Features of Post-Resection Regeneration of Hepatocytes under the Influence of Intrahepatic Administration of Cyanocobalamin

Abstract

Liver resection in most cases remains the only way to achieve an increase in life expectancy and cure of patients with large liver formations. In clinical practice, hepatoprotectors are used to improve metabolic processes of the liver, many of them include vitamin B12, the active form of which is able to stimulate reparative regeneration.The aim of the study was to identify morphological features of hepatocytes and the stroma of the resected liver during intra-organ intraoperative administration of cyanocobalamin to study the effect of vitamin B12 on the proliferative activity of hepatocytes.Material and methods. The experiment was conducted on 36 male Wistar rats. In 3 experimental groups of animals, a typical resection was performed in the volume of 70% of the initial liver mass, in the 1st experimental group, prevention of post-resection liver failure was not carried out, in the 2nd experimental group, 0.9% sodium chloride solution was injected into the preserved liver lobes after resection, in the 3rd-vitamin B12. In the animals of the 1st control group liver resection was not performed. The animals were removed from the experiment in 1, 7 and 14 days after the operation and liver material was selected for morphological examination.Results. A significant increase in the number of binuclear hepatocytes was noted in the animals of the 3rd experimental group in 14 days. When studying liver sections stained with silver impregnation after 14 days of the study, the number of reticular fibers in the animals of the 3rd experimental group was 90% higher than similar parameters in animals of the 1st control group, in the animals of the 1st and 2nd experimental groups it was less than 80%. In the immunohistochemical study, an increase in the proliferative activity of mononuclear and binuclear hepatocytes was observed in all experimental groups by the 14th day of the experiment compared with the 1st day. On the 14th day after the operation, the highest proliferation index was observed in the animals of the 3rd experimental group.Conclusion. The proposed method of intra-organ intraoperative administration of cyanocobalamin increases the proliferative activity of hepatocytes, increases the number of cells entering the mitotic cycle, and restores the structural organization of the liver, its anatomical and functional integrity.