FEATURES OF MORPHOGENESIS OF Actinidia arguta LEAF TISSUES AT MICROCLONAL PROPAGATION

Abstract

The methods of microclonal propagation of Actinidia arguta (Siebold et. Zucc.) Planch ex Mix. (cv. ′Oryginalna′ and cv. ′Don-Juan′) plants by leaf tissues morphogenesis has been proposed. Leaves of aseptic actinidia plants from in vitro culture and leaves of plants grown in the garden were used as explants. When introduced into aseptic culture, the optimal period for the isolation of stem explants was the phase of active growth of actinidia shoots, and the medium - ½MS + 2 mg l-1 IBA and 0.5 mg l-1 BAP. The best culture medium for the morphogenesis of shoots from leaf explants of actinidia was a ½MS nutrient medium with the addition of 3.0 mg l-1 of BAP, В1, В6 vitamins and 12 mg l-1 of iron sulfate Fe2(SO4)3х9Н2О. It was set that the frequency of shoot regeneration depends on the cultivar, plant sex, condition of their cultivation and the part of leaf from which the explant was taken. The maximum regeneration efficiency was 83% with the formation of an average of 4–6 shoots and was achieved for leaf explants of actinidia from aseptic culture. Perlite-based substrate was used for the adaptation of the obtained plants and the efficiency of plant adaptation was 65–80%.