Abstract

Pneumofibrosis is a pathological outcome of pulmonary tissue inflammation. It can complicate any lung and bronchial disorder. The mitochondrial membrane potential reflects functional state of immunocompetent blood cells that influence progression of a chronic inflammatory process. The aim of this work was to study the features of mitochondrial membrane potential (MMP) of immunocompetent blood cells in children with chronic nonspecific lung diseases (CNPD), accompanied by pneumofibrosis. We have examined 79 children with CNPD manifesting with symptoms of focal pneumofibrosis. The group of patients included children with congenital lung malformations (43%), consequences of bronchopulmonary dysplasia (41%), chronic bronchitis (10%), post-pneumonic pulmonary fibrosis (6%). The average age of children was 6.5±1.2 years, including 43 boys (54%) and 36 girls (46%). The comparison group included 46 children with COPD without signs of pulmonary fibrosis, the control group consisted of 30 apparently healthy children. The contents of cells with reduced MMP among lymphocytes, monocytes, and granulocytes in peripheral blood was determined with JC-1 dye, using the BD FACSCalibur instrument and Cell Quest Pro software (Becton Dickinson, USA). The proportion of lymphocytes with reduced MMP in patients with COPD was similar in the children of the main and comparison group, exceeding the indexes of the control group by 1.7 times (p < 0.001). Decreased MMP of granulocytes in children with pneumofibrosis was detected 1.9 times more often than in children with fibrosis-free CNPD cases (p < 0.05), and 3.4 times more common than in children from the control group (p < 0.001). Monocytes with reduced MMM in children with pulmonary fibrosis were detected 2 times more often than in children with COPD without fibrosis (p < 0.05), and 7.3 times more frequent than in the control group (p < 0.001). The changes were more expressed in children during exacerbation of the disease. The revealed features suggest a decreased level of metabolic activity of blood cells, thus, probably, presenting an immunopathogenetic basis for development of pneumofibrosis.

Highlights

  • Анализ первичных данных осуществляли методами вариационной статистики с помощью ППП Statistica 10.0 и Excel 2007

  • Knizhnikova E.V., Research Associate, Group of Medical Environmental Problems of Maternal and Child Health, Laboratory of Integral Studies in Bronchopulmonary and Perinatal Pathology Research, Research Institute of Maternity and Childhood Protection, Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration, Khabarovsk, Russian Federation

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Summary

Краткие сообщения Short communications

ОСОБЕННОСТИ МЕМБРАННОГО ПОТЕНЦИАЛА МИТОХОНДРИЙ ИММУНОКОМПЕТЕНТНЫХ КЛЕТОК КРОВИ У ДЕТЕЙ С ХРОНИЧЕСКИМИ НЕСПЕЦИФИЧЕСКИМИ ЗАБОЛЕВАНИЯМИ ЛЕГКИХ, СОПРОВОЖДАЕМЫМИ ЯВЛЕНИЯМИ ПНЕВМОФИБРОЗА. Евсеева Г.П.1, Книжникова Е.В.1, Супрун Е.Н.1, 2, Супрун С.В.1, Кудерова Н.И.1, Пичугина С.В.1, Полубарцева В.В.1, Евдокимова Т.С.1, Лебедько О.А.1. Целью данной работы явилось изучение особенностей мембранного потенциала митохондрий (МПМ) иммунокомпетентных клеток крови у детей с хроническими неспецифическими заболеваниями легких (ХНЗЛ), сопровождаемыми явлениями пневмофиброза. Лебедько «Особенности мембранного потенциала митохондрий иммунокомпетентных клеток крови у детей с хроническими неспецифическими заболеваниями легких, сопровождаемыми явлениями пневмофиброза» // Медицинская иммунология, 2022. Моноциты со сниженным МПМ у детей с пневмофиброзом выявлялись в 2 раза чаще, чем у детей с ХНЗЛ без фиброза (р < 0,05) и в 7,3 раза чаще по сравнению с группой контроля (р < 0,001), изменения были более выражены у детей в период обострения заболевания.

Материалы и методы
Результаты и обсуждение
Findings
Пул клеток Cell pool
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