FEATURES OF IMMUNE RESPONSE IN PRETERM BABIES WITH BRONCHOPULMONARY DISPLASIA OF VARIOUS SEVERITY

G N Chistyakova,M A Botskovskaya,I I Remizova,S V Bychkova,I A Gazieva,L S Ustyantseva,K P Shamova

doi:10.15789/1563-0625-2019-3-517-526

Abstract

To evaluate the immunity indexes in the children who were born with bronchopulmonary dysplasia (BPD) of varying severity at very early delivery terms (22-27 weeks), dynamic examinations were performed in 35 infants: 17 children were with severe BPD; 18, with mild-severity and moderately severe BPD. The comparison group consisted of seven children born at 22-27 weeks of gestational age without signs of BPD. Relative numbers of lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD16+CD56+, CD4+CD25+), and monocytes (CD14+CD64+, CD14+HLA-DR+) were determined by flow cytometry. The level of cytokines (IL-6, IL-8, IL-4) was measured by enzyme immunoassay technique. The features of immune status in children with BPD of severe, mild and moderate severity were discerned. It was found that the predictors of severe BPD development in the children born at very early terms, are: increased content of IL-8 at birth and at the age of 1 month, reduced level of expression on monocytes (CD64, HLA-DR) on the 1 month of life, and CD14+CD64+cells at 38-40 weeks post conception. The revealed features of immune status in newborns with BPD can be used to assess the effectiveness of the therapy, which requires further research in this direction.

Highlights

Медицинская Иммунология Medical Immunology (Russia)/Meditsinskaya Immunologiya in 35 infants: 17 children were with severe bronchopulmonary dysplasia (BPD); 18, with mild-severity and moderately severe BPD
The comparison group consisted of seven children born at 22-27 weeks of gestational age without signs of BPD
It was found that the predictors of severe BPD development in the children born at very early terms, are: increased content of IL-8 at birth and at the age of 1 month, reduced level of expression on monocytes (CD64, HLA-DR) on the 1 month of life, and CD14+CD64+ cells at 38-40 weeks post conception

Summary

Original articles

Чистякова Г.Н., Ремизова И.И., Устьянцева Л.С., Шамова К.П., Бычкова С.В., Боцьковская М.А., Газиева И.А. С целью оценки показателей иммунитета детей, рожденных в сроке сверхранних преждевременных родов (22-27 недель), с бронхолегочной дисплазией (БЛД) различной степени тяжести, проведено динамическое обследование 35 новорожденных: детей с БЛД тяжелой степени, – БЛД легкой и среднетяжелой степени тяжести. Группу сравнения составили 7 детей 22-27 недель гестационного возраста без БЛД. Выявлены особенности иммунного статуса детей с БЛД тяжелой, легкой и среднетяжелой степени тяжести. Установлено, что предикторами формирования БЛД тяжелой степени у детей, рожденных от сверхранних преждевременных родов, являются повышенное содержания IL-8 при рождении и в возрасте 1 месяца жизни, сниженный уровень экспрессии на моноцитах (CD64, HLA-DR) по достижению 1 месяца жизни и CD14+CD64+ клеток в 38-40 недель постконцептуального возраста. Выявленные особенности иммунного статуса у новорожденных с БЛД могут быть использованы для оценки эффективности проводимой терапии, что диктует необходимость проведения дальнейших исследований.

Материалы и методы

Бронхолегочная дисплазия и иммунитет Bronchopulmonary dysplasia and immunity

Findings

Показатели Parameters