Abstract
The aim of this work is to study the features of the electrical and viscoelastic parameters of erythrocytes in patients with inflammatory bowel diseases (ulcerative colitis, Crohn’s disease, unclassified colitis), taking into account the stage of the disease for possible use in differential diagnosis.The electrical and viscoelastic parameters of erythrocytes were studied using dielectrophoresis in 109 patients with IBD, mean age 37,7 + 11,7 years (50 patients with ulcerative colitis (UC), 41 with Crohn’s disease (CD), 18 with unclassified colitis (UCC) and 53 conditionally healthy, comparable in age and sex with the main groups.Red blood cells of individuals with IBD differed from those in the comparison group by a smaller average diameter, an increased proportion of deformed, spherocytic cells with a changed surface character with a reduced ability to deform, a lower level of surface charge of cells, an altered membrane structure with an increased ability to conduct electric current, prone to destruction and the formation of aggregates (p <0,0001–0,05).Analysis in individual groups with IBD in the acute stage, taking into account the therapy, revealed significant differences between the forms of IBD: in patients with Crohn’s disease, in contrast to patients with UC, red blood cells had lower values of the amplitude of deformation, capacity, dipole moment, and velocity of movement of cells towards electrodes, the proportion of discocytes, polarizability at most of the frequencies of the electric field (p <0,00001–0,05). On the contrary, the summarized indicators of rigidity, viscosity, electrical conductivity, aggregation and destruction indices were higher in CD than in UC (p <0,0001–0,05). CD patients had a greater number of deformed cells with altered surface character (p <0,00001).The features of the electrical and viscoelastic parameters of erythrocytes in patients with differentnosological forms of IBD can be used for the differential diagnosis of ulcerative colitis and Crohn’s disease in case of colon lesions, in the long term — for verification of the diagnosis in unclassified colitis.
Highlights
Анализ в отдельных группах с воспалительных заболеваний кишечника (ВЗК) в стадии обострения с учетом проводимой терапии позволил выявить значимые различия между формами ВЗК: у пациентов с болезнью Крона в отличие от больных с ЯК клетки красной крови имели более низкие значения амплитуды деформации, емкости, дипольного момента, скорости поступательного движения клеток к электродам, доли дискоцитов, поляризуемости на большей части частот электрического поля
K., Odahara, S., Nakamura, M. et al The Fatty Acid Profile of the Erythrocyte Membrane in Initial-Onset Inflammatory Bowel Disease Patients
Summary
The aim of this work is to study the features of the electrical and viscoelastic parameters of erythrocytes in patients with inflammatory bowel diseases (ulcerative colitis, Crohn’s disease, unclassified colitis), taking into account the stage of the disease for possible use in differential diagnosis. The features of the electrical and viscoelastic parameters of erythrocytes in patients with different nosological forms of IBD can be used for the differential diagnosis of ulcerative colitis and Crohn’s disease in case of colon lesions, in the long term — for verification of the diagnosis in unclassified colitis. У пациентов с ВЗК достаточно часто отмечают изменение показателей красной крови, связанные как с прогрессированием самого заболевания [1], так и с проводимой терапией тиопуринами [2], анти-ФНО-α препаратами [3]. Что электрические и вязкоупругие параметры эритроцитов, исследованные методом диэлектрофореза, будут иметь особенности, связанные как с нозологической формой ВЗК, так и стадией заболевания – наличием обострения и ремиссии. Цель работы: изучить особенности электрических и вязкоупругих параметров эритроцитов у пациентов с различными воспалительными заболеваниями кишечника с учетом стадии заболевания для использования в дифференциальной диагностике
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