Features of adsorption of globular proteins by nanoporous silica

Abstract

The adsorption of globular protein macromolecules, bovine serum albumin, and gamma globulin from aqueous solutions at 293 K by nanoporous silica sorbents is investigated. The dependences of the maximum value of adsorption of albumin and gamma globulin from aqueous solutions on the size of nanopores of silica sorbent are obtained. Based on this dependence, the sizes of macromolecular protein structures formed during the adsorption process are determined. A method for evaluating the thickness of adsorbed layers of protein macromolecules on silica surface is proposed. The behavior features of globular proteins in silica nanopores are discussed.