Abstract
Study Objectives – the study of indicators and luminol- lucigenin-dependent chemiluminescence of peripheral blood neutrophils in patients with renal cell carcinoma and bladder cancer prior to the surgery and 10 days after surgery. Held for observation of renal cell carcinoma and bladder cancer patients prior to the (n = 60) and 10 days after surgery (n = 46), aged 45-55 years. The control group consisted of 56 healthy blood donors. Luminol- and lucigenin-dependent chemiluminescence of blood neutrophils estimated De Sole et al. (1983). In patients with renal cell carcinoma and bladder cancer in blood neutrophils showed a change of the system of production of reactive oxygen species in the course of the disease. The observed changes depend on the localization of the tumor process. In patients with renal cell carcinoma changes mainly affect the primary production of reactive oxygen species in patients with bladder cancer early stages of oxidative metabolism and secondary reactive oxygen species. Restoring compensatory metabolic capacity of blood neutrophils in the postoperative period will occur in patients with bladder cancer. The findings clarify the features of the chemiluminescence response of peripheral blood neutrophils from oncourological patients that should probably be used in the development of immune rehabilitation programs in this category of patients in the postoperative period.
Highlights
Held for observation of renal cell carcinoma and bladder cancer patients prior to the (n = 60) and 10 days after surgery (n = 46), aged 45-55 years
In patients with renal cell carcinoma and bladder cancer in blood neutrophils showed a change of the system of production of reactive oxygen species in the course of the disease
In patients with renal cell carcinoma changes mainly affect the primary production of reactive oxygen species in patients with bladder cancer early stages of oxidative metabolism and secondary reactive oxygen species
Summary
Критерии включения в исследование: возраст пациентов 45-55 лет; гистологически верифицированный почечно-клеточный рак либо рак мочевого пузыря; III стадия заболевания (T3N0M0); хирургическое лечение в объеме радикальной нефрэктомии или радикальной цистэктомии; информированное согласие на участие в исследовании. Исследование проведено на базе урологического отделения Красноярского краевого клинического онкологического диспансера в динамике до оперативного вмешательства (n = 60) и через 10 суток после хирургического лечения (n = 46). Люминол- и люцигенин-зависимую ХЛ нейтрофилов периферической крови оценивали методом De Sole et al [7] на биохеми люминесцентном анализаторе CL 3606M (Рос сия). Усиление ХЛ индуцированной зимозаном относительно спонтанной ХЛ оценивали при помощи соотношения S /S зимозан спонтанная и определяли как индекс активации. Хемилюминесценция нейтрофилов при раке почки и мочевого пузыря Chemiluminescence of neutrophils in kidney cancer and bladder программ STATISTICA v. За критический уровень значимости при проверке статистических гипотез при исследовании принят p < 0,05
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