Features indicators of lipid peroxidation and activity of antioxidant enzymes in the lungs in different periods formation of experimental pneumonia

Abstract

Violations of redox processes play a significant role in the pathogenesis of inflammatory due to the increase of oxygen free radicals.The aim of the work was to study oxidant and antioxidant systems in the lungs of guinea pigs in different periods of the formation of experimental pneumonia.The study was carried out on 36 guinea pigs (male) weighing 180–220 grams, divided into 3 groups of 12 animals. I group – intact guinea pigs (control); II group – with the EP on the 6th day; III group – with was EP on the 10th day. EP reproduced by intranasal administration to animals Staphylococcus aureus culture according to Shlyapnyko V. N. and conduthors method. In lung tissue there was determined the content of diene conjugates (DC) V. B. Havrylova, M. I. Myshkorudna method, malondialdehyde (MDA) – there was Korobeynikov E. N. method, superoxide dismutase (SOD) – R. Fried method, catalase (CT) – R. Holmes and C. Masters method.The early period of EP: moderate growth of content DC at 34.8 % and a slight increase of content MDA at 15.3 %, increase SOD activity only at 10.57 % and the likely increase of CT at 12.69 % compared to control group. Late period of EP: further growth DC at 53.86 % and MDA at 37.4 %, SOD activity decrease at 21.37 % and CT at 16.0 % compared with the control.The results of our biochemical studies revealed disruption of lipid peroxidation and antioxidant status in lung tissue at different periods of the formation of the experimental pneumonia.