FEATURES ACCUMULATION OF PHENOLIC COMPOUNDS OF POTENTILLA FRUTICOSA (ROSACEAE) DURING THE DAY

Abstract

Daily changes in the composition and content of phenolic compounds in the aerial parts Potentilla fruticosa L., growing in culture of the Novosibirsk Region, using the method of high performance liquid chromatography (HPLC) was investigated. Six glycosides of flavonol (hyperoside, isoquercitrin, rutin, avicularin, quercitrin and astragalin), two aglycones (quercetin and kaempferol) and tannins (ellagic acid and its glycoside) were identified. Established that phenolic composition of P. fruticosa is the time of day remains constant. The highest total content of phenolic compounds in the leaves (28–29 mg/g of absolutely dry solid matter) and stems (4–4,5 mg/g) of P. fruticosa found in the evening and at night. In the flowers, in contrast, the maximum content of phenolic compounds was observed in the middle of the day (42 mg/g). The level of variability of the total content of phenolic compounds was evaluated as the lowest for leaves and flowers (СV = 10–11%). It was found a threefold increase in free aglycones - quercetin and kaempferol in the leaves with 3 to 6 o'clock in the morning on the background of reduction of the relevant glycosides in 1,5–2,5 times. Maximum accumulation of glycosides of quercetin and minimum content of free quercetin in flowers was observed at 12 o'clock. It is shown predominant accumulation of ellagic compounds quercitrin, component 10, hyperoside, isoquercitrin and rutin in the leaves in the evening and at night, in the flowers – in the middle of the day. The level of variability content of most of the individual components in the above-ground organs of P. fruticosa during the day was estimated as high (СV ≥ 21%).