Abstract
The zebrafish has long been a favourite model for the study of vertebrate development. Here we provide an overview of the current state of knowledge and resources for the study of this fish, with comments on the future direction of zebrafish genomics from Professor Mark Fishman and Dr Stephen Wilson.
Highlights
Zebra®sh (Danio rerio) are small, y3 cm long
$ 3 cm long, translucent, teleost ®sh with black stripes running along their length. $ Eggs are fertilized and embryos develop externally. $ Embryos are clear and observed under the microscope. $ Short generation time (y3 months) is ideal for genetics. $ Diploid, y1700 Mb genome, as 25 chromosomes. $ 25 linkage groups with centromeres have been characterized. $ Radiation Hybrid and genetic maps, YAC and P1 libraries and expressed sequence tags (ESTs) resources available, large-scale mutagenesis project completed, second round under way. $ Comparative studies with other ®sh, in particular medaka and puffer®sh, and other vertebrates, mainly with human and mouse
The initiative is funding the creation of a 0.5±1.0 cM microsatellite map (Shimoda et al, 1999), mapping of theGoodfellow' andEkker' (Hukriede et al, 1999) radiation hybrid panels, which are to be populated with y10 000 expressed sequence tags (ESTs), and a meiotic map (Kelly et al, 2000), which is to include markers in common with the RH maps
Summary
$ 3 cm long, translucent, teleost ®sh with black stripes running along their length. $ Eggs are fertilized and embryos develop (in 2±4 days) externally. $ Embryos are clear and observed under the microscope. $ Short generation time (y3 months) is ideal for genetics. $ Diploid, y1700 Mb genome, as 25 chromosomes. $ 25 linkage groups with centromeres have been characterized. $ Radiation Hybrid and genetic maps, YAC and P1 libraries and EST resources available, large-scale mutagenesis project completed, second round under way. $ Comparative studies with other ®sh, in particular medaka and puffer®sh, and other vertebrates, mainly with human and mouse
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