Feather isotope analysis discriminates age-classes of Western, Least, and Semipalmated sandpipers when plumage methods are unreliable

Abstract

Avian age-class discrimination is typically based on the completeness of the first prebasic molt. In several calidrid sandpiper species, juvenal flight feathers grown on Arctic breeding grounds are retained through the first three migrations. Thereafter, flight feathers are grown annually at temperate migratory stopover sites during the fall or on the subtropical wintering grounds. Standard methods for distinguishing age classes of sandpipers rely on a combination of traits, including body plumage, coloration of protected inner median covert edges, and extent of flight feather wear. We tested the ability of stable hydrogen isotope ratios in flight feathers (Df )t o distinguish young birds in their first winter through second fall from older adults in three calidrid sandpiper species, Western (Calidris mauri), Least (C. minutilla), and Semipalmated (C. pusilla) sandpipers. We compared the apparent reliability of the isotope approach to that of plumage-based aging. The large expected differences in Df values of flight feathers grown at Arctic versus non-Arctic latitudes enabled use of this technique to discriminate between age-classes. We determined Df values of known Arctic-grown feathers from juveniles that grew their flight feathers on the breeding grounds. Flight feather Df values of southward-migrating adults showed bimodal distributions for all three species. Negative values overlapped with species-specific juvenile values, identifying putative second fall birds with high-latitude grown juvenal feathers retained from the previous year. The more positive values identified older adults who grew their feathers at mid- and low latitudes. Importantly, Df analysis successfully identified first-winter and second-fall birds not detected by plumage-based aging. Flight feather wear alone was a poor basis for age classification because scores overlapped extensively between putative second fall birds and older adults. Flight feather hydrogen isotope analysis enables more definitive assignment of age classes when standard plumage methods are unreliable. RESUMEN. El ande is´