Abstract
Objective To investigate the feasible feridex concentration for labeling embryonic stem cells (ESCs) in tracking study in vivo.Methods ESCs were labeled with 0.05,0.10 g/L protamine sulfate-feridex complex respectively,to find out the biological character of ESCs labeled with feridex through electron microscopy and magnetic resonance imaging (MRI) examination.Results With 0.05 g/L protamine sulfate-feridex complex,the feridex labeling efficiency of ESCs was above 91.5%.The number of labeled cells which induced MRI signal changes in vitro was 1 × 105.Conclusion Labeled ESCs with 0.05 g/L protamine sulfate-feridex complex is the optimal choice for transplanted ESCs in vivo. Key words: Feridex; Embryonic stem cells
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