Abstract
In this study, we investigated the potential of thermal hydrolysis pretreatment (THP) to reduce the hydraulic retention times (HRTs) in the anaerobic digestion (AD) of cattle manure (CM). The AD with THP (THP AD) outperformed the control AD by over 1.4 times in terms of methane yield and volatile solid removal, even under the same HRT conditions. Remarkably, even when the THP AD was operated with an HRT of 13.2 d, it performed better than the control AD operated with an HRT of 36.0 d. In THP AD, there was a shift in the dominant archaeal genus responsible for methane generation from Methanogranum (at HRT of 36.0 – 13.2 d) to Methanosaeta (at HRT of 8.0 d). However, decreasing HRT, and applying THP resulted in reduced stability, accompanied by increased inhibitory compounds, and changes in the microbial community. Further confirmation is required to assess the long-term stability of THP AD.
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