Feasibility of the Skin Electrodynamic Introscopy to Assess 2D Acid-Base Balance Dynamics

Abstract

Synchronization is a universal phenomenon that occurs at all levels of the existence of living and inanimate matter. Shortly after enabling monitoring of the electrical impedance landscape of the skin, such phenomena were detected as dynamic clusters with in-phase and anti-phase dynamics. The behavior of these clusters turned out to be very sensitive to various endogenous and exogenous factors and was especially pronounced, for example, at the melanoma areas. Hypothesis of the acid-base balance fluctuations have been put forward as one of the most likely mechanisms for this phenomenon, since hydrogen ions are one of the main factors that determine tissue electrical conductivity. The purpose of this study was to obtain experimental evidence for this hypothesis. The 2D dynamics of glycolysis metabolism was studied on a yeast model as an adequate model of cancer cells. The image sequences reflected the dynamics of processes occurring at the intercellular and intracellular levels, in the form of current values of the 2D active and capacitive components of the impedance in the range of 2 kHz and 1 MHz. As a test factor, a single drop of glucose was used. The revealed effects of clusterization were found similar to those obtained earlier in human experiments. Specifically, these effects were also clearly manifested in the affected region. At the same time, the intercellular medium turned out to be the most rapidly responsive, while the intracellular response occurred with a significant delay but without so marked manifestations of anti-phase behavior. The observed effects are presumably a macroscopic manifestations of quorum sensing mechanisms and cooperative wave processes. We believe that the developed technology can be used as an inexpensive and non-invasive tool for both In vivo and In vitro real-time pH monitoring.