Abstract
Flavan-3-ols are the most abundant flavonoids in diet and the analysis and quantification of flavan-3-ol metabolites could report valuable information to assess their absorption mechanisms and bioavailability. In our research, we have studied the feasibility of the determination of three flavan-3-ols metabolites (3-hydroxyphenylacetic acid, 4-hydroxyphenylacetic acid and syringic acid) in urine samples using parallel factor analysis of fluorescence excitation emission matrices. In the optimised conditions, urine samples were directed analysed after diluting in water, centrifuged and filtered. The procedure was successfully applied to quantify the concentration of the selected flavan-3-ols catabolites in urine samples with recoveries ranging between 85 and 93%. For instance, multivariate analytical figures of merit show low sensitivity and selectivity and LOD between 39μg/L and 53μg/L. The proposed procedure is fast, simple, reliable and cheap method to quantify flavan-3-ols metabolites in urine and constitutes a powerful tool for screening their abundance in different food matrices after consumption.
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