Abstract
Objective: To explore a novel approach for the isolation of human endometriotic stem cells for further study in the stem cell theory of endometriosis. Materials and Methods: A serum-free medium (SFM) was used to isolate the stem cells from ectopic endometrium, eutopic endometrium, and normal endometrium in vitro, and the biological properties including clonogenicity, multipotency, and invasive ability were examined and compared. Results: The ectopic endometrium-derived stem cells cultured in SFM showed higher cloneforming efficiency and sphere formation efficiency (SFE) than eutopic endometrium and normal endometrium, they all can differentiate to endothelial cell and fibroblast, and the ectopic endometrium-derived stem cells also have stronger invasive ability than eutopic endometrium-derived and normal endometrium-derived stem cells. Conclusion: According to the results of the present study, the authors can conclude that human endometriotic stem cells can be isolated in SFM effectively and ectopic endometrium-derived stem cells are probably associated with endometriosis.
Highlights
Endometriosis is a common gynecological disorder with the incidence of 10% to 15% of in reproductive women and 70% to 80% in the women with in fertility and chronic pelvic pain [1, 2], which is characterised by the presence of endometrial tissue outside the uterine cavity
The pathogenesis of endometriosis remains unclear and there are different theories [5, 6] such as endometrium implant, coelomic epithelium metaplasia, immune or genetic factors, and so on, while none of them can explain the mechanism of endometriosis completely
In recent years more and more researchers have focused on the stem cell theory which hypothesizes that endometriosis is caused due to endometrial stem cells retrograde to the pelvic cavity to implant or the other stem cells that differentiate outside of endometrial cavity [7, 8]
Summary
Endometriosis is a common gynecological disorder with the incidence of 10% to 15% of in reproductive women and 70% to 80% in the women with in fertility and chronic pelvic pain [1, 2], which is characterised by the presence of endometrial tissue outside the uterine cavity. The pathogenesis of endometriosis is yet not fully understood and the stem cell theory of endometriosis has obtained increasing focus with the rapid development in the stem cell research field [3, 4]. It remains unclear how the ectopic endometrium-derived stem cells cause endometriosis and it is necessary to isolate the endometriotic stem cells effectively in order to explore the pathogenesis further. Serumfree suspension culture is a common method which is usually used to isolate stem cells without the application experience in endometriotic stem cell. The present study is to attempt to isolate human endometriotic stem cells using serum-free medium
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