Abstract
The cellular and molecular mechanisms responsible for pregnancy-related disorders remain unclear. We investigated the feasibility of using placenta-derived mesenchymal stem cells (MSCs) as a tool to study such pregnancy-related disorders. We isolated and expanded adequate numbers of cells with characteristic features of MSCs from the chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) of human term placental tissues. All placenta-derived MSCs expressed pregnancy-associated C14MC microRNA (miRNA) (miR-323-3p). Interestingly, the placenta-specific C19MC miRNAs (miR-518b and miR517a) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin, but were barely expressed in DB-MSCs of maternal origin. Furthermore, expression levels of placenta-specific C19MC miRNAs in CV-MSCs remained stable during the ex vivo expansion process and across different pregnancy phases (first trimester versus third trimester). High-efficiency siRNA transfection was confirmed in twice-passaged CV-MSCs with little toxicity, and microarray analysis was used to screen for miR-518b target genes. Placenta-derived MSCs, especially CV-MSCs, are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders.
Highlights
We aimed to expand mesenchymal stem cells (MSCs) from different parts of human term placentas including the chorionic plate (CP) and chorionic villi (CV) of foetal origin, and the decidua basalis (DB) of maternal origin (Fig. 1), and to examine the expression of pregnancy-associated miRNAs in MSCs primarily expanded from placental tissues
The origin of the MSCs was examined by microsatellite genotyping of the short tandem repeat (STR) markers D8S1179, TH01, vWs, and amelogenin (Fig. 1d), which revealed the biparental origins of CP-MSCs and CV-MSCs, compared with maternal-origin DB-MSCs
Flow cytometry analysis showed that all CP-MSCs, CV-MSCs, and DB-MSCs expressed the MSC markers CD44, CD73, CD90, and CD105 but not the haematopoietic cell markers CD34 and CD45 (Fig. 2a)
Summary
These results indicate the potential use of placenta-derived MSCs as a tool for investigating the mechanisms behind pregnancy-related disorders. We aimed to expand MSCs from different parts of human term placentas including the chorionic plate (CP) and chorionic villi (CV) of foetal origin, and the decidua basalis (DB) of maternal origin (Fig. 1), and to examine the expression of pregnancy-associated miRNAs in MSCs primarily expanded from placental tissues. We investigated the transfection efficiency of CP-MSCs and CV-MSCs from term placentas with small interfering RNAs (siRNAs), and miR-518b target genes were further screened for by microarray analysis. These preliminary results suggest that CV-MSCs are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
