Abstract
This paper is focused on the feasibility study of parylene as a biocompatible coating for planar electroporation microelectrodes. The planar parallel and the circular interdigitated electrodes are applied in the analysis. The electrodes feature 100 μm width with a 300 μm gap between anode and cathode. The parylene coating thickness was varied in the 250 nm – 2 μm range. The resultant electric field distribution evaluation has been performed using the finite element method. The electrodes have been applied in electroporation experiments with Saprolegnia parasitica. For reference the additional experiments using conventional electroporation cuvette (1 mm gap) have been performed. It has been determined that the parylene coating with hydrophobic properties has limited applicability for the passivation of the planar electroporation electrodes.DOI: http://dx.doi.org/10.5755/j01.ms.23.2.14953
Highlights
Electroporation is a biological cell treatment method based on the application of the pulsed electric field to induce reversible and non-reversible changes in the cell membrane permeability [1 – 4]
The finite element method (FEM) simulation results indicate that the magnitude of the electric field is reduced up to 65 % E0 (E0 – electric field amplitude generated without parylene layer) for the 2 μm parylene coating
The planar electroporation electrodes have been applied in the study and the feasibility of the parylene coating has been analysed
Summary
Electroporation is a biological cell treatment method based on the application of the pulsed electric field to induce reversible and non-reversible changes in the cell membrane permeability [1 – 4]. Conventional electroporation includes a pulsed power generator for high voltage pulse formation and a cuvette for biological cells with integrated electrodes, where a high intensity and defined duration electric field is exerted [5, 6]. In this case the design of the electrodes usually consists of two parallel metal plates (anode and cathode) in direct contact with the cell medium, where a uniform electric field is generated [7]. The influence of parylene layer thickness on the electric field distribution and the respective parameters of electroporation are determined
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
