Abstract
To assess the feasibility of mapping the kinetics and unidirectional fluxes of inorganic phosphate (Pi) to adenosine triphosphate (ATP) reactions in the entire volume of the lower leg muscles using a three-dimensional saturation transfer (ST) phosphorus (31 P) imaging sequence. We imaged the lower leg muscles of five healthy subjects at 7.0 Tesla. The total experimental time was 45 min. We quantified muscle-specific forward reaction rate constants (k'f ) and metabolic fluxes (Vf ) of the Pi-to-ATP reaction in the tibialis anterior, the gastrocnemius, and the soleus. In the tibialis anterior, k'f and Vf were 0.11 s-1 ± 0.03 (mean ± standard deviation) and 0.34 mM s-1 ± 0.10, respectively. In the gastrocnemius, k'f was 0.11 s-1 ± 0.04 and Vf was 0.37 mM s-1 ± 0.11, while in the soleus muscle k'f was 0.10 s-1 ± 0.02 and Vf was 0.36 mM s-1 ± 0.14. Our results suggest that mapping the kinetics and unidirectional fluxes from Pi-to-ATP in both the anterior and posterior muscles of the lower leg is feasible at ultra-high field and may provide useful insights for the study of insulin resistance, diabetes and aging. Magn Reson Med 74:225-230, 2015. © 2014 Wiley Periodicals, Inc.
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