Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma — Next step toward reliable non-invasive prenatal diagnostics

Abstract

We determined the feasibility of universal fetal marker detection in maternal circulation. Using real-time PCR, we compared the levels of fetal ( SRY and hypermethylated RASSF1A) and total ( GLO gene and total RASSF1A) extracellular DNA and fractions of extracellular fetal DNA ( SRY/ GLO vs. hypermethylated RASSF1A/total RASSF1A) in maternal circulation. Sensitivity and specificity reached 100% as the fetal-specific hypermethylated RASSF1A sequence was detected in all 151 examined plasma samples derived from 70 normal pregnancies with a singleton male ( n = 51) or female ( n = 19) fetus sampled throughout gestation and absent in non-pregnant individuals ( n = 29). A strong positive correlation was observed between fetal-derived hypermethylated RASSF1A and SRY ( ρ = 0.66, P < 0.001), total RASSF1A and GLO ( ρ = 0.65, P < 0.001), SRY/ GLO vs. hypermethylated RASSF1A/total RASSF1A ratio ( ρ = 0.62, P < 0.001) in maternal plasma. The results indicate that a universal fetal marker could be useful not only for the confirmation of the presence of fetal cell-free DNA in maternal plasma but could enable quantification of cell-free fetal DNA in pregnancy associated disorders, independently of the sex of the fetus.