Feasibility of digital image colorimetric methods for iron determination in river sediment

Abstract

The current study describes the development of simple, low-cost, and high-throughput digital image colorimetric methods to determine the total iron concentration in river sediment using the spot-test reactions of iron with 1,10-phenanthroline and thiocyanate. The colorimetric assay was done on 96-microzone plates, and a flatbed scanner was applied to acquire the images. The proposed methods offered a linear range from 0.2 to 14.0 mg/L, with a detection limit of 0.11 mg/kg for the 1,10-phenanthroline method, and, for the thiocyanate method, the linear range comprises 2.0–10.0 mg/L, with a detection limit of 0.28 mg/kg. It was observed that both proposed digital image colorimetric methods (1,10-phenanthroline and thiocyanate) yielded statistically similar results to the reference procedures at a 95% confidence level. A standard reference material (NIST 8704) also was utilized for accuracy assessment and the results were statistically equivalent to the certified values within the 95% confidence level. The digital image colorimetric methods can be an alternative method for iron determination in sediment samples, allowing fast sample screening at a low cost.