Abstract
AbstractIn the present work, batch biosorption of Cu, Zn, and Ni ions by Aspergillus sp. was investigated. The effect of initial metal ion concentration (0–500 mg/l), pH (2.0–6.0), inoculum concentration (v/v), and different concentrations of total sugar in cheese whey (2, 4, 6, 8, and 10 g/l) on the biosorption of Cu, Zn, and Ni was studied separately. In the absence of metals and at pH 5, a maximum concentration of 5.62 g/l of biomass was observed. However, a decrease in the concentration of biomass was observed in the presence of Cu, Zn, and Ni. The concentration of Cu, Zn, and Ni was increased from 50 to 500 mg/l, and the maximum specific uptake was found to be 9.4 ± 0.15–62.2 ± 0.20 mg/g, 9.5 ± 0.1–64.0 ± 0.25 mg/g, and 7.2 ± 0.2–29.43 ± 0.08 mg/g, respectively. The biomass concentration increases with the increase in total sugar concentration in cheese whey. Scanning electron microscopy and X-ray Energy Dispersion Analysis depicted the possible cell–metal ions interaction.
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