Feasibility of bioleaching of heavy metals from sediment with indigenous bacteria using agricultural sulfur soil conditioners

Abstract

Sediment bioleaching using a sulfur substrate is a promising approach to the removal of heavy metals. Compared with commercial sulfur powder used as the sulfur substrate, agricultural sulfur soil conditioners may reduce secondary pollution and facilitate the reuse of sediment. This study explored the bioleaching effect of three agricultural sulfur soil conditioners, including sulfur-coated urea, bentonite sulfur, and bio-sulfur, and the bioleaching potential of the indigenous sediment bacteria. The results showed that the sulfur-coated urea had a comparable bioleaching effect with sulfur powder (Ni 35.35%, Cu 74.27%, Zn 69.92%) and the highest maximum bioleaching rate because of the additional nitrogen. The bentonite sulfur leached the least but increased the proportion of the residual state due to its adsorption of heavy metal. Similar changes to the microbial flora structure and bioleaching mechanism were found with the use of sulfur powder, sulfur-coated urea, and bentonite sulfur as the bioleaching substrate. There was no significant difference between the indigenous bacteria and the sludge-enriched bacteria in the bioleaching effect except for bio-sulfur, which only performed well with the sludge-enriched bacteria. In the absence of inoculum, the bio-sulfur hindered the bioleaching process due to high levels of organic matter. This study provides insights into the practical application of bioleaching heavy metal removal technology from the perspective of sulfur substrate selection.