Feasibility Assessment of Parathyroid Hormone Adsorption by Using Polysaccharide-Based Multilayer Film Systems.

Ruey-Shin Juang,I-Chi Lee,Xing Su

doi:10.3390/polym13132070

Abstract

Chronic kidney disease (CKD) is a systemic disorder that combines complex bone and mineral abnormalities. The high level of parathyroid hormone (PTH) in the blood causes irreversible renal dysfunction and cardiovascular disease. Therefore, it is necessary to reduce level of PTH in the blood of patients with uremic state. In this study, chitosan and heparin were chosen to form polysaccharide-based multilayer films based on their antibacterial ability, good biocompatibility and hemocompatibility. In addition, a previous study has revealed that PTH is a heparin/polyanion binding protein because of the similarity of heparin to the cell surface proteoglycans. Subsequently, the surface properties including thickness, surface hydrophobicity and surface charge of a series of multilayer films were analyzed. The PTH adsorption rate of a series of multilayer films was also assessed. The results revealed that the optimizing condition is (CHI/HEP)2.5 and 60 min in both PBS only and PBS with the addition of bovine serum albumin, which demonstrated the specific adsorption of PTH on the materials. Furthermore, the hemolysis test also revealed that (CHI/HEP)2.5 shows good blood compatibility. It is considered that polysaccharide-based multilayer films may provide an alternative for the surface modification of hemodialysis membranes and equipment.

Highlights

Hyperparathyroidism is a disease characterized by excessive secretion of parathyroid hormone (PTH), which is a common finding in Chronic kidney disease (CKD) patients with renal failure [1,2]
The PEM films were prepared with chitosan adsorption on a glass coverslip with plasma treatment, forming a physically coated chitosan film, followed by heparin adsorption generating1 as shown in Scheme 1A
It is shown that the film thickness increased with the number of layers, where 20 nm of chitosan was the thinnest and roughly 90 nm of4 .5 was the thickest

Summary

Introduction

Chronic kidney disease (CKD) is a global and public problem and generally progresses into chronic renal failure. Hyperparathyroidism is a disease characterized by excessive secretion of PTH, which is a common finding in CKD patients with renal failure [1,2]. Several studies tried to evaluate the calcium, phosphate and PTH concentrations in dialysis populations and analyzed the associations from abnormal mineral metabolism to cardiovascular disease [3]. The mechanism is complex, it is considered that high levels of calcium phosphate product and PTH are associated with an increased relative risk of death and cardiovascular-specific mortality in patients with kidney disease [4]. Moderate to severe hyperparathyroidism (PTH concentrations ≥ 600 pg/mL) is especially associated with an increase in the relative risk of death [4]. PTH is considered a uremic toxin responsible for many of the abnormalities of the uremic state and bone disease

Methods

Results

Conclusion