Abstract
This study pioneered the production of biomethane from E. coli fermenter effluent through anaerobic fermentation using wastewater treatment sludge with the aim of enhancing production by optimizing various factors affecting the anaerobic digestion process, including the Food-to-microorganism (F⋅M-1) ratio, pH level, and substrate Chemical Oxygen Demand (COD). Peak biomethane yield of 156 ± 2.63 ml⋅g CODadded-1 and volatile solids (VS) reduction up-to 89% was observed for the optimum condition of F∙M-1 ratio 2.6, pH 6.5 and COD 50 g⋅l-1. Results indicates adjusting the pH to 6.5 (for F⋅M-1 2.6) enhanced the inherent methanogenic homeostasis which counteracted the inhibitory effects of ammonia. This led to 22% increase in biomethane production compared to corresponding control sample. 16S rRNA analysis was conducted to study the diversity in the microbial communities of the samples. Kinetic study also employed modified Gompertz equation to model the cumulative biomethane production (R2 > 0.946).
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
