Feasibility analysis of inactivating influenza A(H1N1) virus using UVC robot in classroom environment

Abstract

BackgroundStarting from 2009, H1N1 has been one of the respiratory diseases that afflict the global population. Concurrently, due to the influence of COVID-19, it has become widely accepted that preventing the virus's spread necessitates personal protection measures and disinfection in public spaces. ExperimentsThis study conducted two experiments. In the classroom experiment, six UVC dose test points were calibrated to test whether the UVC dose at each testing point met the standards for inactivating IAVs and the time required to meet the standards. In the simulated classroom experiment, seven square slides made of IAVs were placed. After 10minutes of robot movement, irradiated sterile square slides were made into suspension and injected into chicken embryos. Cultivate chicken embryos and conduct IAVs testing. ResultsClassroom experiment has shown that 5 testing points can meet the standards for inactivating IAVs(3mJ/cm2), with a required time of 80minutes, 40minutes, 15minutes, 5minutes and 10minutes. The UVC dose for testing points that do not meet the standards in 80minutes is only 0.5mJ/cm2. The simulation classroom experiment outcomes revealed that 99.99% of IAVs were deactivated. Furthermore, this study established both a desktop control group and a chair arm control group, both of which yielded identical results, indicating an inactivation logarithm of IAVs≥4log. ConclusionThe study presented that IAVs on the surface of an object can be effectively and rapidly deactivated at an irradiation density of 1.8mW/cm2. Meanwhile, the study provides evidence of the feasibility of using the GXU robot to inactivate IAV in a classroom environment.