Fe3 O4 nanozyme coating enhances light-driven biohydrogen production in self-photosensitized Shewanella oneidensis-CdS hybrid systems.

Abstract

Solar-driven biohybrid systems that produce chemical energy are a valuable objective in ongoing research. However, reactive oxygen species (ROS) that accompany nanoparticle production under light radiation severely affect the efficiency of biohybrid systems. In this study, we successfully constructed a two-hybrid system, Shewanella oneidensis-CdS and S. oneidensis-CdS@Fe3 O4 , in a simple, economical, and gentle manner. With the Fe3 O4 coating, ROS were considerably eliminated; the hydroxyl radical, superoxide radical, and hydrogen peroxide contents were reduced by 66.7%, 65.4%, and 72%, respectively, during light-driven S. oneidensis-CdS hydrogen production. Shewanella oneidensis-CdS@Fe3 O4 showed a 2.6-fold higher hydrogen production (70h) than S. oneidensis-CdS. Moreover, the S. oneidensis-CdS system produced an additional 367.8μmol g-dcw-1 (70h) of hydrogen compared with S. oneidensis during irradiation. The apparent quantum efficiencies of S. oneidensis-CdS and S. oneidensis-CdS@Fe3 O4 were 6.2% and 11.5%, respectively, exceeding values previously reported. In conclusion, a stable nanozyme coating effectively inhibited the cytotoxicity of CdS nanoparticles, providing an excellent production environment for bacteria. This study provides a rational strategy for protecting biohybrid systems from ROS toxicity and contributes to more efficient solar energy conversion in the future. This article is protected by copyright. All rights reserved.