Abstract
Lung cancer is the leading cause of cancer-related mortality worldwide, characterized by uncontrolled proliferation and metastasis of lung cancer cells. Tumor angiogenesis plays a key role in proliferation and metastasis in cancers, and is an essential component in microenvironment. It has been reported that long non-coding RNA FBXL19-AS1 plays an oncogenic role in colorectal cancer. However, the molecular mechanism of FBXL19-AS1 in lung cancer has not been fully elucidated. In the present study, we found that FBXL19-AS1 expression was up-regulated in lung cancer tissues and cell lines. FBXL19-AS1 knockdown inhibited cell proliferation, migration, invasion, and angiogenesis in lung cancer cells. Molecular mechanism exploration uncovered that FBXL19-AS1 acted as a molecular sponge of miR-431-5p and that RAF1 was a downstream target of miR-431-5p in lung cancer. Moreover, there was a negative association between miR-431-5p expression and FBXL19-AS1 or RAF1 expression in tumor tissues. Through rescue experiments, we discovered that overexpression of RAF1 partially rescued FBXL19-AS1 knockdown-mediated inhibition of angiogenesis and progression in lung cancer. Together, these results indicated that FBXL19-AS1 was involved in progression and angiogenesis in lung cancer by targeting miR-431-5p/RAF1 axis, which provided a new insight into the therapeutic strategies of lung cancer.
Highlights
Lung cancer is the leading cause of cancer-related mortality worldwide, with the highest incidence in males and the third in females [1]
The results showed that FBXL19-AS1 expression was markedly up-regulated in lung cancer tissues compared with adjacent normal tissues (Figure 1A)
Previous studies revealed that Long non-coding RNA (lncRNA) participate in many cancers, such as gastric cancer, bladder cancer, and lung cancer [20–22]
Summary
Lung cancer is the leading cause of cancer-related mortality worldwide, with the highest incidence in males and the third in females [1]. Lung cancer accounts for more than 1/4 of all cancer deaths, with only 10–15% of 5-year overall survival [2]. Tumor angiogenesis plays a key role in cancers, and it is an essential component in microenvironment of tumor growth and metastasis [3]. Tumor blood vessels provide oxygen and nutrients for the metabolism of tumor tissues, allowing rapid growth of tumor and providing a base for distant metastasis of the tumor [4]. There are many studies on various biomarkers in lung cancer, but the potential molecular regulation mechanisms of these biomarkers are still not very clear. It is greatly important to find out the biological targets related to anti-angiogenesis in lung cancer and better understand the molecular mechanism of their regulation
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