Abstract
Abstract 1. 1. Total lipids were extracted from the ameboid sperm of Ascaris suum and separated into neutral, glyco- and polar lipid fractions by silicic acid column chromatography and preparative TLC. 2. 2. The fatty acid composition of fractions was determined by gas-liquid chromatographic (GLC) analysis of fatty acid methyl esters. 3. 3. Six saturated: (12:0, 14:0, 16:0, 18:0, 20:0, 22:0) and sixteen unsaturated (14:1, 14:2, 16:1, 16:2, 18:1, 18:2, 20:1, 20:2, 20:4, 20:5, 22:1, 22:3, 22:4, 22:5, 22:6, 24:1) fatty acids were identified in the sperm extract. One saturated (18:0; 12%) and five unsaturated (18:1, 18:2, 20:1, 20:2, 22:1; 74%) fatty acids represented 86% of the total. 4. 4. The major neutral lipid component of the sperm migrated as a free fatty acid when analyzed by thin-layer chromatography (TLC). This component was purified by preparative TLC; GLC analysis indicated that it contained five major fatty acids identified as 18:0, 18:1, 18:2, 20:1 and 20:2.
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