Abstract
Oily seed extracts were used as research samples in Analytical Laboratory of Raw Materials and Plant Products. Supercritical carbon dioxide extraction on an industrial scale was used to prepare extracts from raw plant materials, usually berry seeds: blackcurrant, raspberry, strawberry, and chokeberry. Gas chromatography analysis was performed on Agilent equipment with single quadrupole mass spectrometer detector and split/splitless injector. Fatty acids (FAs) were determined using indirect method, where they were converted to corresponding methyl esters (FAMEs) during trimethylsulfonium hydroxide solution reaction. Comparing the mass spectrum and retention time peak, more than 30 compounds in different oily extracts were qualitatively identified. Quantification of individual fatty acids was based on two different methods. Firstly, it was based on the received peak area, and the results were normalized without correction factor. Secondly, the quantification was based on external calibration curve, for 34 identified fatty acids. The method was validated, and the results, e.g., linearity, precision, limit of detection (LOD), and limit of quantification (LOQ), were presented.
Highlights
IntroductionThey have complicated matrix, which is problematic during analysis (matrix effect) (Yaroshenko and Kartsova 2014); they contain many valuable, healthy substances known as bioactive compounds including flavonoids (polyphenols), phenolic and hydroxycinnamic acids, lignans, vitamins, carotenoids, monoterpenes, and lipids (phytosterols, tocopherols, and saturated and unsaturated fatty acids)
They have complicated matrix, which is problematic during analysis (Yaroshenko and Kartsova 2014); they contain many valuable, healthy substances known as bioactive compounds including flavonoids, phenolic and hydroxycinnamic acids, lignans, vitamins, carotenoids, monoterpenes, and lipids
The pro-health approach to fatty acids forced the producers of oils and food, as well as companies that deal with this subject, to check the level and content determination of individual fatty acids and their isomers
Summary
They have complicated matrix, which is problematic during analysis (matrix effect) (Yaroshenko and Kartsova 2014); they contain many valuable, healthy substances known as bioactive compounds including flavonoids (polyphenols), phenolic and hydroxycinnamic acids, lignans, vitamins, carotenoids, monoterpenes, and lipids (phytosterols, tocopherols, and saturated and unsaturated fatty acids). Agricultural production and the berry fruit industry have a significant position in Poland (Nawirska et al 2007; Agricultural Market Agency 2014; Kraciński 2014). In 2004–2013, berry fruit collection, in Poland (Fig. 1), were 5–11% of the fruit products in EU. In 2013, they were at the record level of 4.13 million tons, about 29% higher than average yields in 2004–2012 (Agricultural Market Agency 2014; Kraciński 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
