Abstract
Volatile energy costs and environmental concerns have spurred interest in the development of alternative, renewable, sustainable, and cost-effective energy resources. Environment-friendly processes involving microbes can be used to synthesize advanced biofuels. These fuels have the potential to replace fossil fuels in supporting high-power demanding machinery such as aircrafts and trucks. From an engineering perspective, the pathway for fatty acid biosynthesis is an attractive route for the production of advanced fuels such as fatty acid ethyl esters, fatty alcohols, and alkanes. The robustness and excellent accessibility to molecular genetics make the yeast Saccharomyces cerevisiae a suitable host for the purpose of bio-manufacturing. Recent advances in metabolic engineering, as well as systems and synthetic biology, have now provided the opportunity to engineer yeast metabolism for the production of fatty acid-derived fuels and chemicals.
Highlights
Volatile energy costs and environmental concerns have motivated the development of sustainable, renewable, and cost-effective alternative energy sources that have reduced pollution emissions or carbon footprints (Fortman et al, 2008)
Given that the addition of exogenous fatty acids was essential for high fatty acid ethyl esters (FAEEs) production, the enhancement of fatty acid biosynthesis would be necessary for the increased de novo synthesis of FAEE from sugar-based carbon sources
After enhancing the fatty acid biosynthesis pathway and introducing a malic enzyme from Mucor circinelloides for improved NADPH supply, the fatty alcohol titer increased to 98 mg/L (Runguphan and Keasling, 2014), which showed the potential of S cerevisiae as a platform for fatty alcohol production
Summary
Volatile energy costs and environmental concerns have motivated the development of sustainable, renewable, and cost-effective alternative energy sources that have reduced pollution emissions or carbon footprints (Fortman et al, 2008). Fatty acid biosynthesis has attracted significant attention for production of highly reduced biofuels and chemicals with high energy densities (Lennen and Pfleger, 2013). It has received increased attention as a host for the synthesis of fatty acid-derived biofuels and chemicals (Li et al, 2014; Runguphan and Keasling, 2014).
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