Abstract
BackgroundConjugated linoleic acids (CLA) are in focus of dairy cattle research because of its milk fat reducing effects. Little is known about the impact of CLA on immune function in dairy cows. Therefore, in the present study we investigated the effects of a long term supplementation of dairy cows with CLA on the fatty acid profile of peripheral blood mononuclear cells (PBMC) and their proliferation ex vivo.ResultsThe supplementation of dairy cows with either 100 g/d of a control fat preparation (CON, n = 15), 50 g/d of the control fat preparation and 50 g/d CLA supplement – containing 12.0% cis-9, trans-11 and 11.9% trans-10, cis-12 CLA of total fatty acid methyl esters – (CLA-50, n = 15) or 100 g/d of the CLA supplement (CLA-100, n = 16) did not influence the major fatty acids (C18:0, C16:0, cis-9 C18:1, cis-9, cis-12 C18:2, cis-5, cis-8, cis-11, cis-14 C20:4) in the lipid fraction of PBMC. The proportion of trans-10, cis-12 CLA of total fatty acids was increased in both CLA supplemented groups, but there was no effect on the cis-9, trans-11 isomer. Furthermore, the proportion of trans-9 C18:1 and cis-12 C24:1 was reduced in the CLA-100 group. The mitogen stimulated cell proliferation was not influenced by CLA feeding.ConclusionCLA supplementation influenced the FA profile of some minor FA in PBMC, but these changes did not lead to differences in the mitogen induced activation of the cells.
Highlights
Conjugated linoleic acids (CLA) are in focus of dairy cattle research because of its milk fat reducing effects
Fatty acid profile of peripheral blood mononuclear cells (PBMC) The main fatty acids (FA) occurring in PBMC were C18:0, C16:0, cis-9 C18:1, cis-9, cis-12 C18:2 and cis-5, cis-8, cis-11, cis14 C20:4 (Table 1)
CLA supplementation did not change the proportions of these FA significantly, but there was a tendency of increasing C16:0 when CLA was supplemented
Summary
Conjugated linoleic acids (CLA) are in focus of dairy cattle research because of its milk fat reducing effects. In the present study we investigated the effects of a long term supplementation of dairy cows with CLA on the fatty acid profile of peripheral blood mononuclear cells (PBMC) and their proliferation ex vivo. Conjugated linoleic acids (CLA) are a group of positional isomers of linoleic acid, which are characterized by conjugated double bonds They are intermediate products in the biohydrogenation of unsaturated fatty acids (FA) by microorganisms in the rumen [1]. The SI of splenocytes from the same animals were decreased following CLA supplementation [12] It is unknown, if the effects are similar in pluriparous cows and over a longer supplementation period and if the supplementation changes the FA profile of immune cells, which might have further downstream effects. Effects on immune cells were evaluated by cell proliferation assays using PBMC and the FA profile of PBMC was analyzed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
