Abstract
Several factors influence an individual’s susceptibility in inter-individual lipid changes and its role in the onset of type-2 diabetes mellitus (T2DM). Considering the above fact, the present investigation focuses on determining the association between fatty acid desaturase 2 (FADS2) rs174575 (C/G) polymorphism, circulating lipid levels and susceptibility to type-2 diabetes mellitus. As per the inclusion and exclusion criteria a total of 429 subjects (non-diabetic-216; diabetic-213) were recruited for the study. Glycemic and lipid profile status were assessed using commercially available kits. Based on the previous reports SNP rs174575 of fatty acid desaturase gene (FADS2) was selected and identified using the dbSNP database. The amplified products were sequenced by means of Sanger sequencing method. Lipid profile status and apolipoprotein levels revealed statistically significant difference between the groups. Three models were assessed namely, recessive model (CC vs CG + GG), dominant model (CC + CG vs GG) and additive model (CC vs CG vs GG). The recessive model, displayed a statistically significant variations between the circulating lipid levels in T2DM. The multivariate model with genotype (G allele carriers), triglyceride (TG) and insulin served as a predictive model. The study results potentiate the functional link between FADS2 gene polymorphism, lipid levels and type-2 diabetes mellitus.
Highlights
Type 2 diabetes mellitus (T2DM) is referred as an epidemic, increasing at an alarming rate
Joshi SR reported that Asian Indians consists of smaller body size but suffer from central obesity and higher subscapular-to-triceps skin fold ratio when compared with their British counterparts, the term thin-fat Indian[7]
The present study aims to find an association between fatty acid desaturase 2 gene polymorphism and circulating lipid levels in type-2 diabetes
Summary
Type 2 diabetes mellitus (T2DM) is referred as an epidemic, increasing at an alarming rate. Joshi SR reported that Asian Indians consists of smaller body size but suffer from central obesity (higher waist to hip ratio) and higher subscapular-to-triceps skin fold ratio when compared with their British counterparts, the term thin-fat Indian[7] It reflected in triacylglycerol (TG) and higher plasma nonesterified fatty acid (NEFA) concentrations, hyperinsulinemia (with fasting), higher insulin resistance (IR) and post-glucose challenge s tates[7,8]. Correlation between fatty acid desaturase (FADS) genetic variants (encoding rate-limiting enzymes for PUFA synthesis) with desaturase activities and blood lipids are reported[19] When considering these results it can be concluded that the desaturation pathway appears to be extremely important for lipid homeostasis in the human body. The present study aims to find an association between fatty acid desaturase 2 gene polymorphism and circulating lipid levels in type-2 diabetes
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