Abstract
Gas-liquid chromotographic analyses using lipid extracts of Ips calligraphus (Germar) in its various developmental stages showed the presence of 8:0, 10:0, 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, and 19:0 fatty acids, plus one unidentified compound. The rearing medium contained hark phloem of Pinus elliottii Engelmann var. elliottii and was qualitatively similar in fatty acid composition to beetle tissues. The major fatty acids in all developmental stages of I. calligraphus were 16- and 18-carbon acids, and 16:0 and 18:1 acids each comprised about 30–40% of the total. Both 16:0 and 18:1 acids decreased in each sex during adult maturation. Unsaturated acids together comprised about 50% of total in prepupae, 64% in mature virgin females, and 62% in stage-I (nonfeeding) larvae. The major unsaturated acid in adult beetles and stage-I larvae was 18:1. Percents of 18:2 and 18:3 acids were higher in adult virgin females than in adult males of the same age, a sexassociated difference that increased during adult maturation. Both 18:2 and 18:3 acids increased from the prepupal to 15-day-old adult stages, but the percent of 18:2 was highest (22%) and 18:3 lowest (trace) in stage-I larvae.
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