Abstract
Data is provided to show the detailed fatty acid and lipidomic composition of normal and tumor rat colon tissues. Rats were fed either a Western fat diet or a fish oil diet, and half the rats from each diet group were treated with chemical carcinogens that induce colon cancer (azoxymethane and dextran sodium sulfate). The data show total fatty acid profiles of sera and of all the colon tissues, namely normal tissue from control rats and both normal and tumor tissues from carcinogen-treated rats, as obtained by gas chromatography with mass spectral detection. Data from lipidomic analyses of a representative subset of the colon tissue samples is also shown in heat maps generated from hierarchical cluster analysis. These data display the utility lipidomic analyses to enhance the interpretation of dietary feeding studies aimed at cancer prevention and support the findings published in the companion paper (Effects of fish oil supplementation on prostaglandins in normal and tumor colon tissue: modulation by the lipogenic phenotype of colon tumors, Djuric et al., 2017 [1]).
Highlights
Fatty acid and lipidomic data in normal and tumor colon tissues of rats fed diets with and without fish oil
Half the rats in each diet group were treated with a chemical carcinogen to induce colon tumors
Lipid extraction was done by the Folch method prior to total fatty acid analyses by Gas-chromatography with mass spectral detection (GC–MS)
Summary
Tables and heat maps Two methods of lipid analysis were employed: Gas-chromatography with mass spectral detection (GC–MS) and liquid chromatography with tandem mass spectral detection (LC–MS–MS). Half the rats in each diet group were treated with a chemical carcinogen to induce colon tumors. Normal and tumor colon tissues were flash frozen prior to extraction and analysis. Lipid extraction was done by the Folch method prior to total fatty acid analyses by GC–MS. Lipid extraction of select colon tissues for lipidomic analyses was done using the Bligh–Dyer method. The data show fatty acids and lipids in four groups of rats: rats randomized to receive a fish oil or Western diet, and a chemical carcinogen or not. Lipidomic profiles show the effects of a fish oil diet in normal and tumor colon tissues.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
