Abstract
Pyrolysis mass spectrometry (Py-MS) and in situ thermal hydrolysis-methylation (THM) of whole bacteria with tetramethylammonium hydroxide (TMAH) produced diagnostic fatty acid methyl ester (FAME) profiles for Pediococcus damnosus, P. dextrinicus, and Lactobacillus brevis. Bacterial fatty acids were analyzed from bacteria in beer without the need for a fatty acid extraction or chromatography procedure. FAME profiles were obtained from bacteria in beer at a concentration of 107 colony forming units (CFU)/ml of beer. The major FAME profiles observed for P. damnosus were palmitic acid methyl ester (C16:0 ME), oleic acid methyl ester (C18:1 ME), and cyclopropyl nonadecanoic acid methyl ester (cyC19:0 ME). P. dextrinicus produced mainly C18:1 ME, and L. Brevis showed both C16:0 ME and C19:0 ME. The analysis procedure produced fatty acid profiles with a total analysis time of 15 min/sample.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
