Fate, toxicity and effect of triclocarban on the microbial community in wastewater treatment systems

Abstract

Triclocarban (TCC), one of the typical antimicrobial agents, is a contaminant of emerging concern commonly found in high concentration in water environments. However, the fate and toxicity of TCC in wastewater treatment systems remain poorly understood. Here, we investigated how TCC impacts chemical oxygen demand and inorganic nitrogen transformation in a hydrolytic anaerobic-anoxic/oxic process. In the anaerobic section, the transformation of TCC was dominated by reductive dechlorination and supplemented by two amid bonds hydrolysis. In the anoxic and oxic sections, the hydrolysis of amid bonds dominated. The toxicity was reduced after the treatment (IC50 from 0.09 to 0.54). TCC inhibited NH4+-N removal in the anaerobic section and led to the NO3--N accumulation (2.84–4.13 mg/L) after treatment, with the abundance of N-removal bacteria decreased by 6%. Furthermore, the original ecological niche was gradually replaced by TCC-resistant/degradative bacteria, formating new microbial modules to resist the TCC stress. Importantly, fourteen genera including Methanosaeta, Longilinea, Dokdonella and Mycobacterium as potential bioindicators warning TCC and its intermediates were proposed. Overall, this study provides new insights into the fate of TCC in biological wastewater treatment systems and suggests a great importance for TCC control to ensure the health and resilience of ecosystems.