Fate of 13C labelled root and shoot residues in soil and anecic earthworm casts: A mesocosm experiment

Abstract

Earthworms are known to have a major impact on organic matter dynamics in soils. The precise dynamics of carbon incorporation and/or decomposition in soil under the influence of earthworms still need to be investigated. In a mesocosm experiment, the fate of Ryegrass root and shoot litter was monitored in the soil, in the presence and absence of anecic earthworms Lumbricus terrestris L. Residues were13C labelled and deposited onto the soil surface. Incorporation of 13C in surface casts and in the 0–20 and 40–60cm soil layers was monitored 1, 2, 4, 8, 24 and 54weeks after adding labelled litter. Organic carbon content and δ13C values were obtained for all samples, allowing the determination of the percentage of carbon derived from labelled litter (Clab). Roots and shoots were incorporated in the 0–20cm soil layer during the year of experiment, Clab reaching 11.4% of the soil organic carbon after 54weeks. On the contrary, no significant contribution from labelled residues was observed in the 40–60cm layer. Roots decomposed at a slower rate compared to shoots. Litter incorporation was observed in casts from the very first weeks of experiment (Clab from 34.8 to 51.4% after 2weeks). In the soil, a significant effect of earthworms on the Clab was detected after 24weeks. Earthworms accelerated root and shoot decomposition in the soil. They also enhanced, in the presence of shoot residues, the decomposition of the organic matter originally present in the soil. However, after one year, earthworms smoothed the difference between residue types in casts and to a lesser extent in soil, revealing their capacity to enhance the decomposition of both roots and shoots.