Abstract
BackgroundTrueperella pyogenes is a worldwide known bacterium causing mastitis, abortion and various other pyogenic infections in domestic animals like ruminants and pigs. In this study we represent the first case report of three unusual fatal infections of Grey Slender Lorises caused by Trueperella pyogenes. Meanwhile, this study represents the first in-depth description of the multilocus sequence analysis (MLSA) on T. pyogenes species.Case presentationThree Trueperella pyogenes were isolated from three different Grey Slender Lorises, which died within a period of two years at Frankfurt Zoo (Frankfurt am Main - Germany). The three Grey Slender Loris cases were suffering from severe sepsis and died from its complication. During the bacteriological investigation of the three cases, the T. pyogenes were isolated from different organisms in each case. The epidemiological relationship between the three isolates could be shown by four genomic DNA fingerprint methods (ERIC-PCR, BOX-PCR, (GTG)5-PCR, and RAPD-PCR) and by multilocus sequence analysis (MLSA) investigating four different housekeeping genes (fusA-tuf-metG-gyrA).ConclusionIn this study, we clearly showed by means of using three different rep-PCRs, by RAPD-PCR and by MLSA that the genomic fingerprinting of the investigated three T. pyogenes have the same clonal origin and are genetically identical. These results suggest that the same isolate contaminated the animal’s facility and subsequently caused cross infection between the three different Grey Slender Lorises. To the best of our knowledge, this is the first epidemiological approach concentrating on T. pyogenes using MLSA.
Highlights
Trueperella pyogenes is a worldwide known bacterium causing mastitis, abortion and various other pyogenic infections in domestic animals like ruminants and pigs
In this study, we clearly showed by means of using three different rep-PCRs, by Randomly amplified polymorphic DNA (RAPD)-PCR and by multilocus sequence analysis (MLSA) that the genomic fingerprinting of the investigated three T. pyogenes have the same clonal origin and are genetically identical
These results suggest that the same isolate contaminated the animal’s facility and subsequently caused cross infection between the three different Grey Slender Lorises
Summary
The several genetic markers of the presented MLSA approach showed clearly that the three T. pyogenes originating from Grey Slender Lorises and the two T. pyogenes reference strains belong to three different clonal complexes, respectively. The results of the present investigation which represent the first detailed epidemiological study of T. pyogenes of this origin clearly indicated that all three T. pyogenes which contributed with other potentially pathogenic bacteria to the septicemia of the three lorises, respectively shared a clonal origin. TE performed the initial examination of the isolates at the Veterinary Examination Office and helped in drafting the manuscript. OS and AB carried out the sequencing conducted the molecular investigations (DNA isolation, PCR assays and data interpretation). CL, AA and AB performed the critical revision of the manuscript and gave important intellectual advice for approval. All authors read and approved the final manuscript. Author details 1Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, D-35392 Gießen, Germany. Author details 1Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstraße 81, D-35392 Gießen, Germany. 2Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany. 3Landesbetrieb Hessisches Landeslabor, Schubertstraße. 60, D-35392 Gießen, Germany. 4Zoologischer Garten Frankfurt am Main, Bernhard-Grzimek-Allee 1, D-60316 Frankfurt, Germany. 5Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurter Straße. 85-91, D-35392 Gießen, Germany. 6Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hannover, Germany
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
