Abstract
The cultural conditions of Chlorella ellipsoidea were studied with special reference to lipide accumulation. It was ascertained that there is a close relation between the lipide content and the nitrogen content of cells. Cultivation with the nitrogen-free medium was applied to produce nitrogen deficient cells, and in this culture were studied the effects of light intensity, algal density and depth of algel suspension upon lipide accumulation. The annual yield of fat by mass-culture was roughly estimated. In order to learn the relation between fat accumulation and carbohydrate metabolism in nitrogen-deficient Chlorella cells, the change of the fractions of carbohydrate during the progress of nitrogen-deficiency was studied in the first place. Some remarkable behaviour of certain fractions was observed. The change of chlorophyll and carotinoid content were also investigated.
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