FAT SOLUBLE VITAMINS AND FATTY ACIDS COMPOSITION OF BLACK SEA CYSTOSEIRA BARBATA

Veselina Panayotova,Mona Stancheva

doi:10.12955/cbup.v1.58

Veselina Panayotova, Mona Stancheva

Open Access

https://doi.org/10.12955/cbup.v1.58

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Abstract

Brown alga Cystoseira barbata is the most widely distributed seaweed in the Black Sea. There is limited information about fat soluble vitamins content and fatty acids composition of this specie from Bulgarian Black Sea coast. The aim of this study was to determine fat soluble vitamins, pigments, total lipid and fatty acid composition of Cystoseira barbata. Fat soluble vitamins (vitamin E and D), pigments (β-carotene and astaxanthin) and total cholesterol were analyzed simultaneously using HPLC/UV/FL system equipped with RP analytical column. Sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Brown seaweed Cystoseira barbata contained high amounts of α-tocopherol and β-carotene. Lipids were extracted by following the method of Bligh and Dyer. The residual lipid fraction was methylated using base-catalyzed transmethylation with methanolic potassium hydroxide. Fatty acid composition was analyzed by GC/MS. Cystoseira barbata was rich in linoleic (C18:2n6) and eicosopentaenoic acid (C20:5n3) although total lipid content was generally low. High levels of α-tocopherol correlate with high levels of polyunsaturated fatty acids. As an antioxidant α-tocopherol preserves tissue PUFA from oxidation.

Highlights

Seaweeds have been used since ancient times as food, fodder, fertilizer and as source of medicine
Because of the huge and renewable biomass and the fact that many of them could be cultivated in the sea on a large scale, seaweeds are a potential source of fatty acids for biotechnology and a dietary source of essential fatty acids (Khotimchenko et al, 2002)
The total lipids content of Cystoseira barbata accounted for 3.35±0.58g per 100 g on a dry weight basis

Summary

Introduction

Seaweeds have been used since ancient times as food, fodder, fertilizer and as source of medicine. Nowadays seaweeds represent an inexhaustible source of the raw materials used in pharmaceutical, food industries, medicine and cosmetics. They are nutritionally valuable as fresh or dried vegetables, or as ingredients in a wide variety of prepared foods. Marine algae are rich in PUFAs of the n-3 and n-6 series, which are considered essential fatty acids for humans and animals. Some of these FAs (20:3n-6, 20:4n-6, 20:5n-3) have high biological activity and are converted into eicosanoids. Because of the huge and renewable biomass and the fact that many of them could be cultivated in the sea on a large scale, seaweeds are a potential source of fatty acids for biotechnology and a dietary source of essential fatty acids (Khotimchenko et al, 2002)

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