Fat Restriction At Various Developmental Periods Cause Different Modulations On The Lingual Fatty Acid Binding Proteins

Abstract

Background: The origin of food preferences can be associated with early food experience and lingual fatty taste receptors have a vital role in fat sensation. Objective: To determine the effect of fat restriction during maternal and/or maturation periods on oro-sensoral perception of fats, fatty taste preference and expression of CD36 and GPR120 in circumvallate papillae of male offspring Sprague Dawley rats. Methods: In our study, rats were exposed to control diet (C)(13% energy-from-fat) and low-fat diet (LFD)(3% energy-from-fat) during the gestation- lactation (21 days-21 day) and maturation (120 days) periods. Experimental groups were C/C (n=6), C/LFD (n=8), LFD/C (n=6) and LFD/LFD (n=8). Animal weight, energy and fat intake of the offsprings were followed during the study. The fat perception of the rats were measured by behavioral tests. The plasma insulin and leptin levels were determined with ELISA. Blood glucose and triglyceride levels were measured by metabolic analyzer. The protein and mRNA expressions of CD36 and GPR120 in the circumvallate papillae were determined by western blotting, immunofluorescence, and RT-PCR. Results: This study has demonstrated that C/LFD group rats have significantly increased preference to 2% rapeseed oil solution than C/C group (p<0.05). Consistently, lingual CD36 and GPR120 expressions are significantly lower in C/LFD group (p<0.05). In addition, LFD administration during maternal period in LFD/C and LFD/LFD groups caused to significantly increased expression of lingual CD36 and GPR120 (p<0.05). Conclusion: This study has been contributed to identify the developmental dysregulations of the oro-sensoral signaling pathways and abnormal fatty taste perception.