Abstract
It has been suggested that large amounts of ketonic bile acids may be present in portal venous blood. We have therefore determined the approximate concentration of 3-oxo-, 7-oxo-, and 12-oxo-bile acids (monoketonic bile acids) in human peripheral and portal circulation. These compounds were converted into the corresponding 3alpha-, 7alpha-, and 12alpha-hydroxy bile acids by treatment with sodium borodeuteride, thus increasing the molecular weight of each bile acid formed by one mass unit. The ratio between deuterated and nondeuterated bile acid was determined by combined gas-liquid chromatography-mass spectrometry with use of selected ion monitoring. From the ratio obtained and from the concentration of unlabeled bile acid, determined by isotope dilution-mass spectrometry, the approximate concentration of the different ketonic bile acids could be calculated. This method underestimates 3-oxygenated bile acids by 4-8%, 7-oxygenated bile acids by 2-3%, and 12-oxygenated bile acids by about 25%. The approximate concentration of monoketonic 3,7-oxygenated bile acids was found to be 0.08 +/- 0.02 and 0.37 +/- 0.25 micro mol/l in the peripheral venous serum and the portal venous serum, respectively. The approximate concentration of monoketonic 3,12-oxygenated bile acids was found to be 0.07 +/- 0.02 and 0.32 +/- 0.12 micro mol/l in the peripheral venous serum and the portal venous serum, respectively. The approximate concentration of monoketonic 3,7,12-oxygenated bile acids was found to be 0.03 +/- 0.01 and 0.14 +/- 0.05 micro mol/l in the peripheral venous serum and in the portal venous serum, respectively. The total concentration of the ketonic bile acids constituted only 9 +/- 1% and 8 +/- 3% of the nonoxidized bile acids in the peripheral venous serum and in the portal venous serum, respectively. Thus it seems less likely that the portal inflow of ketonic bile acids is of significant physiological importance under normal conditions.-Björkhem, I., B. Angelin, K. Einarsson, and S. Ewerth. Fasting levels of monoketonic bile acids in human peripheral and portal circulation.
Highlights
It has been suggested that large amounts of ketonic acids in portal blood whenassayed by the Sa-hydroxybile acids may be present in portal venous blood
It was considered important to determine at Supplementarykey words serumbileacids cholic acid chenodeoxycholic acid deoxycholic acid mass fragmentography portal blood least the approximate level of the ketonic bile acids in portal venous and peripheral venous blood
In view ofthe methodologicaldifficulties inthe direct assayof the ketonic bile acids in serum by gas-liquid chromatography, we have reduced them with sodium
Summary
The subjects of the study were five femalesand one male(ages 32-79 years) suffering from cholelithiasis andadmittedfor elective cholecystectomy. The gallbladder was functioning at the time of the study. There was no history of previous liver diseaseor of common bile duct stones, and all patients displayed normal liver function tests
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