Abstract
The report suggests determining the microvascular flow index (MFI), the perfused vessel density (PVD) and the percentage of perfused vessels (PPV). For the MFI a grid is used dividing the screen into four quadrants, and the vessels are scored according to observed flow: 0 = none, 1 = intermittent, 2 = sluggish, 3 = continuous. For the PVD and the PPV, three equidistant horizontal and vertical lines are drawn and a different score is used: absent, intermittent, present (for details see [1]).
Highlights
The recently published report ‘How to evaluate the microcirculation’ [1] should be praised for standardizing analysis of human microcirculation data
For the microvascular flow index (MFI) a grid is used dividing the screen into four quadrants, and the vessels are scored according to observed flow: 0 = none, 1 = intermittent, 2 = sluggish, 3 = continuous
I propose using the same grid for the MFI, the percentage of perfused vessels (PPV) and the perfused vessel density (PVD)
Summary
The recently published report ‘How to evaluate the microcirculation’ [1] should be praised for standardizing analysis of human microcirculation data. See related research by De Backer et al, http://ccforum.com/content/11/5/R101 This standardization will enable better comparison between studies. Having worked extensively with both orthogonal polarization spectral (OPS) and sidestream dark field (SDF) imaging and most analysis software, I feel the proposed analysis is extremely useful but is time consuming.
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