Abstract
Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.
Highlights
Hantaviruses are enveloped RNA viruses belonging to the family Hantaviridae (OrderBunyavirales) [1]
The limit of detection (LOD) of the Thailand orthohantavirus (THAIV) rtRT-PCR assays were similar for both systems used with the ability to detect up to 3.45 FFU/mL (Table 2)
We demonstrated that the LOD of our assay was identical to the one of the pan-hantavirus nested RT-PCR developed by Klempa et al [10]
Summary
Hantaviruses are enveloped RNA viruses belonging to the family Hantaviridae (OrderBunyavirales) [1]. Hantaviruses are enveloped RNA viruses belonging to the family Hantaviridae Hantavirus genome encompasses three negative single-strand segments. Large (L), Medium (M) and Small (S) encode respectively for RNA-dependent RNA polymerase (RdRp), two external glycoproteins (Gn and Gc) and the nucleocapsid (N) protein. Each protein is coded from a gene with a single Open Reading Frame (ORF). An additional ORF encoding a non-structural (NSs) protein is added at the S-segment of some hantavirus species carried by certain rodent species [2]. The 3' and 5' terminal sequences of hantavirus genome are more conserved and complementary and can form a hairpin structure. This structure distinguishes viruses belonging to the order Bunyavirales [3]
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