Fast screening of some flavonoids content in raw plant materials: opportunities of 1H NMR spectroscopy

Г А Калабин ,Vasilii Vasil’Ev,В А Ивлев ,В А Бабкин

doi:10.1051/e3sconf/202016902006

Г А Калабин , Vasilii Vasil’Ev + Show 2 more

Open Access

https://doi.org/10.1051/e3sconf/202016902006

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Abstract

Environmental monitoring and assessment of the prospects for extracting biologically active substances (BAS) from various types of plant biomass requires the development of simple and fast methods for measuring their content in raw materials. A new approach for measuring the content of various flavonoids groups in plant raw material using 1H NMR spectroscopy has been developed, which allows to characterize its resource capabilities and study the effects on their composition different environmental factors without complex sample preparation and standard samples.

Highlights

The concept of effective environmental management is not limited by the main environmental imperative - the conservation of environmental biodiversity
Assessment of the content of biologically active substances in biomass should be based on environmentally friendly quantitative analytical methods free from complex sample preparation, derivatization, and the need for standard samples
Using of the 5-OH groups proton signals, it is possible to identify the flavonol glycosides (FG) in the extracts of plant origin and to determine their content, because signals of aromatic and aliphatic protons do not appear in this region of the spectrum

Summary

Introduction

The concept of effective environmental management is not limited by the main environmental imperative - the conservation of environmental biodiversity. Its economic component is the rational consumption of natural raw materials. Trees and higher plants are important renewable resources of the biosphere, the processing methods of which should be non-waste and produce cellulose, construction and composite materials. Scientifically-based their use involves obtaining a wide range of food, medical and technical products. Assessment of the content of biologically active substances in biomass should be based on environmentally friendly quantitative analytical methods free from complex sample preparation, derivatization, and the need for standard samples. Taking into account the multicomponent nature of the metabolite extracts, NMR spectroscopy meets these requirements due to sufficient sensitivity, informativity and standardless quantitative measurements

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