Fast quantification of fatty acid profile of intact fish by intermolecular double‐quantum coherence 1H‐NMR spectroscopy

Abstract

Intermolecular double quantum coherence (iDQC) 1H‐nuclear magnetic resonance spectroscopy (1H NMR), which serves as a complementary method in the analyses of fatty acids, is used to investigate the intact salmon muscle and the whole zebra fish. The spectra of fatty acids of the intact salmon muscle, whose resonances are overlapped with metabolites peaks in the conventional NMR spectra, can be resolved in the presence of severe intrinsic structural inhomogeneity without sample pretreatments and special NMR accessories, such as those for high‐speed sample spinning. For improving the practicability of the iDQC method, a localized module is combined with the iDQC method so that the fatty acids of the whole zebra fish can be detected non‐invasively. All the iDQC results are verified by the extraction NMR spectra. In addition, fatty acid composition of the salmon muscle is quantitatively analyzed based on the iDQC and extraction NMR spectra. The calculated results from these two methods are in good agreement. Therefore, the iDQC method may serve as a feasible one‐step and fast screening method for fish quality analyses and lipid inspections of other biological tissues.Practical applications: The intermolecular doublequantum coherence (iDQC) can be an appropriate lipid detecting method of tissues when the samples are not suitable for chemical extraction. The iDQC with a localized module may be more practical than magic angle spinning for applications in in vivo and in situ NMR experiments, in which samples are not allowed to spin.Fatty acids of the whole zebra fish can be identified by iDQC method when PRESS with water suppression fails to produce high‐resolution spectrum.