Abstract
Rapid and high-yielding ion-exchange chromatographic and chromatofocusing methods using fast polymer liquid chromatography ( FPLC) have been developed for plant myrosinase, β-thioglucoside glucohydrolase ( EC 3.2.3.1), isoenzyme studies. The preparative FPLC methods showed a mean recovery of 85% for six myrosinase preparations from three different plant sources. FPLC chromatofocusing and analytical isoelectric focusing were used to demonstrate the presence of different mixtures of myrosinase isoenzymes in seeds of various crucifers. The stability of the isoenzymes was investigated. The proposed method of plant isoenzyme analysis is briefly discussed in relation to methods previously used for myrosinase isolation and characterization, and studies of glucosinolate catabolism.
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